By Tomaz Jarm, Peter Kramar
This quantity offers the complaints of the first international Congress on Electroporation and Pulsed electrical Fields in Biology, medication and nutrition & Environmental applied sciences (WC2015). The congress came about in Portorož, Slovenia, through the week of September sixth to tenth, 2015. The medical a part of the Congress lined diverse elements of electroporation and comparable applied sciences and integrated the next major issues:
· software of pulsed electrical fields expertise in meals: demanding situations and opportunities
· electric impedance dimension for evaluation of electroporation yield
· Electrochemistry and electroporation
· Electroporation meets electrostimulation
· Electrotechnologies for foodstuff and biomass treatment
· meals and biotechnology applications
· In vitro electroporation - simple mechanisms
· Interfacial behaviour of lipid-assemblies, membranes and cells in electrical fields
· Irreversible electroporation in scientific use
· scientific functions: electrochemotherapy
· clinical functions: gene therapy
· Non-electric field-based actual equipment inducing mobile poration and improved molecule transfer
· Non-thermal plasmas for nutrients safeguard, environmental functions and clinical treatments
· PEF for the nutrition undefined: basics and applications
· PEF proce
ss integration - complicated procedure chains and strategy mixtures within the nutrition industry
· Predictable animal models
Pulsed electrical fields and electroporation applied sciences in bioeconomy
· Veterinary clinical applications
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Additional resources for 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies: Portorož, Slovenia, September 6 –10, 2015
Rols. 2011. First explanations for differences in electrotransfection efficiency in vitro and in vivo using spheroid model. Int J Pharm. 38. , and M. P. Rols. 2013. Progress and Prospects: The Use of 3D Spheroid Model as a Relevant Way to Study and Optimize DNA Electrotransfer. Current Gene Therapy 13:175-181. 39. P. and Gibot, L. 2015. Efficient In Vitro Electropermeabilization of Reconstructed Human Dermal Tissue. J Membrane Biol 15. 40. Tokman M. et al. 2013. 11;8(4) 41. Polak A. et al. 2014.
Micron 48:26-33. , J. Teissie, and M. P. Rols. 2002. Direct visualization at the single-cell level of electrically mediated gene delivery. Proc Natl Acad Sci U S A 99:1292-1297. 19 23. Rosazza, C. et al. 2011. The actin cytoskeleton has an active role in the electrotransfer of plasmid DNA in mammalian cells. Mol Ther 19:913-921. 24. Rosazza, C. et al. 2011. Cholesterol implications in plasmid DNA electrotransfer: Evidence for the involvement of endocytotic pathways. Int J Pharm. 25. Faurie, C. et al.
Free DNA is perhaps also internalized through electropores. For gene expression to occur (5-6), DNA has to cross the cytoplasm toward the nucleus. Our study suggests (5) actin related motion that can be in the form of (5a) actin polymerization that pushes the DNA, free or in vesicles (actin rocketing) and/or (5b) transport via the myosins (in both directions). We observe (6) microtubules related motion which can mean (6a) transport via kinesin and dynein, (6b) DNA interaction with oppositely directed motors and with (6c) several motors of the same type (6d).