Animal Cell Technology. Products of Today, Prospects for by R. E. Spier, J. B. Griffiths, W. Berthold

By R. E. Spier, J. B. Griffiths, W. Berthold

Most crucial for conceivable and filthy rich animal mobile know-how is the success of profitable items hence developing profit for sufferers and credibility for the industrial appeal of this fairly younger know-how. The papers awarded during this quantity deal with the newest matters and glance to destiny advancements within the fields of animal phone expertise. very important subject matters thought of in those displays contain downstream processing and regulatory safeguard facets. The twelfth ESCAT assembly lawsuits proceed to supply an entire assessment of this significant subject and may be a useful reference resource for these occupied with the construction and use of animal cells

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Iwamoto,K. and Kitano,K. Appl. Microbiol. Biotechnol. , Iwamoto,K. and Kitano,K. Cytotechnolog y 1989, 2, 9 Katsuta,H. and Takaoka,T. Methods in Cell Biol. , Sato,S. and Fujiyoshi,N. , Hitotsumachi,S . and Tsukamoto,K. Biochem. Biophys. Res. Commun. , Takano,S. , Morioka,H. and Shibai,H. In: Biotechnology of mammalian cells (Ed. Umeda,M. ) Jpn. Sei. , Sasada,R. , Honjo,T. and Kitano,K. Cytotechnolog y 1990,4,29 Shirahata,S. , Tachibana,H . and Murakami,H. In Animal Cell Culture and Production of Biologicals (Ed.

The specific production rate of six closely related recombinan t CHO cell lines measure d in attached cultures were higher, lower, or similar to that measure d in suspensio n cultures of the same cell line. Likewise the specific production rate measure d in static cultures was found to be of restricted use during the selection of recombinan t NSO cell lines. The optimum number of cell lines to select by different criteria has been estimated . Large differences in the productivity of closely related recombinant cell lines even after two rounds of cloning has been observed .

Stingl, G. L. Gene Therapy for Cancer: The Utilit y of Transferrinfection in generating "Tumor Vaccines". Gene 1993, in press. , Cotten, M. L. Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine/DN A complexes: Towards a synthetic virus-like gene transfer vehicle. Proc. Natl. Acad. Sei. USA 1992, 89, 7934-7938. , Mechtler, K. and Wagner, E. Gene transfer into hepatocyte s using asialoglycoprotei n receptor mediated endocytosis of DNA complexed with an artificial tetra-antennar y galactose ligand.

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